南寧師范大學(xué)蘇煒&林陳想等：具有近紅外響應(yīng)的卟啉自組裝納米光敏劑實(shí)現(xiàn)高效I型光動(dòng)力和光熱治療
2025-05-22 15:09:46
作者：中國(guó)科學(xué)材料 來(lái)源：中國(guó)科學(xué)材料
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具有近紅外光響應(yīng)的I型光敏劑對(duì)氧的依賴(lài)性較低，可實(shí)現(xiàn)有效的電子轉(zhuǎn)移，在缺氧微環(huán)境光動(dòng)力治療中具有極大的應(yīng)用潛力。
近日，南寧師范大學(xué)蘇煒教授、林陳想博士、廣西中醫(yī)藥大學(xué)李培源研究員和北京科技大學(xué)姜建壯教授等人在Science China Materials發(fā)表研究論文，通過(guò)5,10,15,20-四(4-氨基苯基)卟啉（TAPP）與N-芐氧羰基-L-亮氨酸（Cbz-Leu）的共組裝策略，成功構(gòu)建了超分子納米光敏劑TAPP@Cbz-Leu。
本文要點(diǎn)
1) 在近紅外光（808 nm）照射下，由于TAPP與Cbz-Leu之間的非共價(jià)的緊密結(jié)合，可誘導(dǎo)光生電子從Cbz-Leu向三重態(tài)TAPP轉(zhuǎn)移，生成TAPP·-，因 此，TAPP@Cbz-Leu可以顯著生成O2·-。此外，TAPP@Cbz-Leu在808 nm近紅外光照射下具有優(yōu)異的光熱性能。2) 體內(nèi)實(shí)驗(yàn)表明，TAPP@Cbz-Leu對(duì)MRSA感染的動(dòng)物傷口模型具有良好的抗菌效果，并能有效促進(jìn)傷口愈合。
本研究首次通過(guò)簡(jiǎn)單共組裝策略實(shí)現(xiàn)了II型光敏劑向I型光敏劑的轉(zhuǎn)化，為開(kāi)發(fā)新型近紅外響應(yīng)的光療材料的構(gòu)建提供了新思路。
Figure 1. Schematic illustration of the conversion of type II photosensitizer (TAPP) triggered by 660?nm light to type I photosensitizer (TAPP@Cbz-Leu) triggered by 808?nm NIR light.Figure 2. UV-vis absorption spectroscopy of (a) TAPP@Cbz-Leu with different TAPP and Cbz-Leu stoichiometric ratios, and (b) TAPP, Cbz-Leu, the mixed solution of TAPP and Cbz-Leu in DMSO, and TAPP@Cbz-Leu in water. (c) Fluorescence intensities of TAPP in DMSO and TAPP@Cbz-Leu in water. (d) TEM image of TAPP@Cbz-Leu. (e) Size distribution in water detected by DLS. (f) FT-IR spectra of TAPP, Cbz-Leu and TAPP@Cbz-Leu.
Figure 3. (a) ROS generation of TAPP@Cbz-Leu in water with irradiation, detected by DCFH. (b) ROS generation of TAPP, ICG and TAPP@Cbz-Leu in water with irradiation. (c) 1O2 production of TAPP@Cbz-Leu in water with irradiation detected using SOSG. (d) O2·- production of TAPP@Cbz-Leu in water with irradiation detected using DHE. (e) O2·- production of TAPP and TAPP@Cbz-Leu in water upon irradiation. (f) O2·- production of TAPP and TAPP@Cbz-Leu upon irradiation under normal and hypoxic conditions. (g) ESR spectra of O2·- trapped by DMPO in TAPP@Cbz-Leu with or without irradiation. (h) Energy levels of TAPP@Cbz-Leu and TAPP. (i) Nanosecond transient absorption spectra of TAPP and TAPP@Cbz-Leu dispersed in water (0.5?mM). All the irradiation is 808?nm, 0.5?W/cm2.
Figure 4. (a–c) Viability and (d) representative pictures of MRSA, S. aureus and E. coli following treatment with TAPP@Cbz-Leu. (e) Live/Dead staining of MRSA treated with TAPP@Cbz-Leu. (f) Detection of ROS generation of TAPP@Cbz-Leu via DCFH probe. (g) SEM images of S. aureus and E. coli with and without treatment with TAPP@Cbz-Leu. Error bars represent the mean ± SD (n ≥ 3). All the irradiation is 808?nm, 0.5?W/cm2.
Wei Su (蘇煒) received his BS and PhD degrees from Shandong University, and then he worked at North Carolina State University as a postdoc. He joined Nanning Normal University in 2008 and is currently a full professor. His work focuses on porphyrin and phthalocyanine materials and their applications in photocatalysis.
Lei Cen (岑磊) received his Master’s degree under the supervision of Prof. Wei Su and Dr. Chenxiang Lin at Nanning Normal University. His research interest mainly focuses on phototherapy antibacterial of porphyrin and phthalocyanine self-assembled nanomaterials.
Dong Yang (楊棟) is currently studying for a Master’s degree under the supervision of Prof. Wei Su and Dr. Chenxiang Lin at Nanning Normal University. His research focuses on preparation of functional hydrogels and antimicrobial phototherapy.
Chenxiang Lin (林陳想) received her BS degree from Guangxi Normal University in 2015 and PhD degree from University of Science and Technology Beijing in 2021. Then, she worked at Guangxi Key Laboratory of Natural Polymer Chemistry and Physics of Nanning Normal University as a lecturer. Her research focuses on the design and synthesis of porphyrin and phthalocyanine functional materials for phototherapy antibacterial and photocatalytic hydrogen production.
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